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Ukulinganisa iimeko zomzimba kunceda abaphandi bafumane izibophelelo zesinyithi

Abaphandi baye baqulunqa indlela yokuchonga iimolekyuli ezincinci ezibopha ii-ion zetsimbi. Iiion zesinyithi zibalulekile kwibhayoloji. Kodwa ukuchonga ukuba zeziphi iimolekyuli—ingakumbi zeziphi iimolekyuli ezincinci—ezi ion zentsimbi zidibana nazo kunokuba lucelomngeni.

Ukwahlula i-metabolites ukuhlalutya, iindlela eziqhelekileyo ze-metabolomics zisebenzisa i-solvents ye-organic kunye ne-pH ephantsi, enokubangela ukuba i-metal complexes ihlukane. U-Pieter C. Dorrestein weYunivesithi yaseCalifornia eSan Diego kunye noogxa bakhe bafuna ukugcina iikhompleksi kunye ukuze zihlalutywe ngokulinganisa iimeko zendalo ezifumaneka kwiiseli. Kodwa ukuba basebenzise iimeko zefiziyoloji ngexesha lokwahlulwa kweemolekyuli, bekuya kufuneka baphinde balungise iimeko zokwahlula kwimeko nganye yefiziyoloji abafuna ukuyivavanya.

Endaweni yoko, abaphandi baphuhlisa indlela yamanqanaba amabini azisa iimeko ze-physiological phakathi kokuhlukana kwe-chromatographic eqhelekileyo kunye nohlalutyo olukhulu lwe-spectrometric (Nat. Chem. 2021, DOI: 10.1038 / s41557-021-00803-1). Okokuqala, bahlula isicatshulwa sebhayoloji besebenzisa ichromatography yolwelo olusebenzayo oluqhelekileyo. Emva koko bahlengahlengisa i-pH yokuhamba ephuma kwikholamu yechromatographic ukulinganisa iimeko ze-physiological, zongezwa ii-ion zetsimbi, kwaye zahlalutya umxube nge-mass spectrometry. Baqhube uhlalutyo kabini ukufumana i-spectra yobunzima beemolekyuli ezincinci kunye nangaphandle kweentsimbi. Ukuchonga ukuba zeziphi iimolekyuli ezibopha isinyithi, basebenzise indlela yokubala esebenzisa iimilo zencopho yokulinganisa unxibelelwano phakathi kwembonakalo yeenguqulelo ezibotshiweyo nezingadityaniswanga.

Enye indlela yokuqhubela phambili ukulinganisa iimeko ze-physiological, u-Dorrestein uthi, iya kuba kukongeza ukugxila okuphezulu kwee-ion ezifana nesodium okanye i-potassium kunye nokugxila okuphantsi kwesinyithi esinomdla. “Iba ngumfuniselo wokhuphiswano. Ngokusisiseko iya kukuxelela, kulungile, le molekyuli phantsi kwezo meko inokuthambekela ngakumbi ukubopha isodiyam kunye nepotassium okanye le ntsimbi inye yahlukileyo oyongezileyo, ”utshilo uDorrestein. "Singafaka iintsimbi ezininzi ezahlukeneyo ngaxeshanye, kwaye sinokuqonda ngokwenene ukhetho kunye nokukhetha kulo mongo."

Kwizicatshulwa zenkcubeko ezivela kwi-Escherichia coli, abaphandi bachonge iikhompawundi zokubopha intsimbi eyaziwayo njenge-yersiniabactin kunye ne-aerobactin. Kwimeko ye-yersiniabactin, bafumanise ukuba inokubophelela i-zinc.

Abaphandi bachonge iikhompawundi ezibophelela isinyithi kwiisampulu zintsonkothile njengezinto eziphilayo ezinyityilisiweyo ezivela elwandle. “Leyo yenye yezona sampuli zintsonkothileyo endakha ndazijonga,” utshilo uDorrestein. “Mhlawumbi intsonkothe ​​njengeoli ekrwada, ukuba ayintsonkothanga ngakumbi.” Indlela echonge i-domoic acid njenge-molecule yokubopha ubhedu kwaye iphakamisa ukuba ibophe i-Cu2 + njenge-dimer.

"Indlela ye-omics yokuchonga zonke i-metabolites ezibophelela isinyithi kwisampulu iluncedo kakhulu ngenxa yokubaluleka kwe-biological metal chelation," u-Oliver Baars, ofunda i-metabolites ebophe isinyithi eveliswa zizityalo kunye ne-microbes kwiYunivesithi yaseNorth Carolina State. imeyile.

"UDorrestein kunye nabantu osebenza nabo babonelela ngovavanyo oluhle, olufunekayo kakhulu, lokuvavanya ngcono ukuba ingaba yintoni indima ye-ion yensimbi kwiseli," u-Albert JR Heck, uvulindlela kuhlalutyo lwe-mass spectrometry kwiYunivesithi yase-Utrecht, ubhala nge-imeyile. "Inyathelo elilandelayo elinokuthi libe kukukhupha i-metabolites phantsi kweemeko zemveli ukusuka kwiseli kwaye uhlukanise ezi nazo phantsi kweemeko zemveli, ukubona ukuba zeziphi i-metabolites ezithwala ii-ion zentsimbi zeselula."

Iindaba zeMichiza kunye nezobuNjineli
ISSN 0009-2347
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